The unique feature of this virtual instrument is that it is thoroughly integrated with a computercontrolled patch clamp amplifier epc 9 to allow estimation of equivalent circuit parameters based upon calibrated admittance measurements. Electrophysiology systems blm planar lipid bilayer workstation. Epc 10 usb single patch clamp amplifier bronjo medi. An integrated four pole low pass bessel filter provides flexible signal conditioning. Patch clamp amplifiers from single channels to large macroscopic recordings. We use igor software to do our analysis and make publication ready. Imaging extension for patchmaster, potmaster and chartmaster. There are 16 additional digital outputs and 16 digital inputs available at the rear panel of the epc 10 usb. Epc 9 the fully computer controlled patchclamp amplifier with builtin interface board. This amplifier enables an investigator to identify a single cell in a preparation by its characteristic spike train, then inject dye. Increased current injection capability by a factor of 5. The epc 9 or epc 10 is the ideal patch clamp amplifier for capacitance measurements because all the relevant parameters are under its control.
Current clamp recordings with the epc 10 patch clamp. Digital control of filter settings, gain and compensation networks allows the admittance of the signal source at the amplifiers input to be determined based on the amplifiers own calibration. A dll dynamic link library is available to allow access of the system from a custom written application. The patchserver is an addon tool for automating a manual patch clamp setup.
This allows this amplifier to be a true fast current clamp amplifier with no instability. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system. The epc 10 usb double, triple, and quadro patch clamp amplifiers are the optimal instruments for performing double or triple patch experiments. Dear all, im looking to purchase a new patch clamp amplifier. Epc 10 usb heka patch clamp amplifiers pc505b patch clamp amplifier pc501a patch clamp amplifier. Extracellular, intracellular and patch clamp amplifier. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster or tida software is equivalent to a fully equipped setup, which includes a. Cc circuitry of the epc 10 amplifier current injection path via the feedback resistor of the patch clamp headstage. How much it will cost on the average to obtain and set up. Pdf design of the epc9, a computercontrolled patch. Elsevier journal of neuroscience methods 56 1995 195202 design of the epc 9, a computercontrolled patchclamp amplifier. A headstage must be selected and included when purchasing a patch clamp amplifier.
Filter 2 usable for current or voltage signal more. The patchclamp amplifier is a ubiquitous tool for characterizing ionchannel activity. Epc 10 usb double triple quadro patch clamp amplifiers. Fast and slow capacitive transients were cancelled by the compensation circuit of the epc 10 amplifier. Axon instruments patchclamp amplifiers molecular devices. Apply the cfast fastcapacitance correction, wait for the seal to stabilize between 510 g. Winwcp is a data acquisition and analysis program for handling signals from wholecell electrophysiological experiments. A patch clamp amplifier and corresponding headstage are also included when purchasing patch clamp recording systems. Epc 10 usb heka patch clamp amplifiers pc505b patch clamp amplifier pc501a patch clamp amplifier need support.
If a dongle is not attached to the computer then the programs will run in demo mode. Heka is now shipping epc 10 usb patch clamp amplifiers of hardware revision t. Used heka elektronik epc 10 single patch clamp amplifier unit. Apparatus suitable for conducting a method according to any of claims 1 to 8, which comprises the probe, means for measuring andor controlling the distance of the probe from a surface, means for bringing the probe into contact with the surface, and a patch clamp amplifier.
The master software family with its members patchmaster, fitmaster, potmaster, pgmaster, chartmaster and epcmaster have been updated to version 2x90. This paper describes various user interfaces that have been implemented, including a highlevel programming interface, a user interface based on the postscript language, and. Continuing the tradition of providing the worlds best electrophysiology amplifiers, the epc 10 usb double, triple, and quadro patch clamp amplifiers are the optimal instruments for performing double or triple patch experiments. The output of either patch clamp amplifier is scaled down by a factor of 10 before input to the lockin amplifier by a 10 k. The system allows a highspeed threshold detection epc 10 usb patch clamp amplifier sends out a voltage signal that helps the user to determine when the tip makes contact with the cell membrane. It is a logical successor to the classical epc 7, retaining all of its features but adding a number of capabilities.
The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is equivalent to a fully equipped recording setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a full featured data acquisition and analysis system. Currents were recorded using an epc10 patchclamp amplifier heka, lambrecht, germany and patchmaster acquisition software heka. Pdf design of the epc9, a computercontrolled patchclamp. The epc 9 patch clamp amplifier is a digitally controlled analog device for recording currents in membrane patches and in small cells.
Heka is now shipping epc 10 usb patch clamp amplifiers. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster or tida software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system. Click here to learn about the new features and bugfixes and download the new version. The letters epc stand for extracellular patch clamp, the method introduced by neher and sakmann 1976 for recording the currents in a small patch of membrane under voltage clamp conditions. The epc 800 usb can be controlled with any data acquisition interface and can be supported, not only by heka software, but.
A software lockin amplifier slia was developed to allow hightimeresolution measurement of membrane capacitance as a singlecell assay of exocytosis. Q series electrode holders str, port, ag wire sizes 1. Heka elektronik single patch clamp amplifier epc 10 epc10 for. Want to know more about our quality products or need assistance in technical support. Epc 10, heka,patch clamp,singapore,amplifiers bronjo medi. Im looking to purchase a new patch clamp amplifier.
Patch clamp amplifiers, auto planer oocyte scitech. Heka is very proud to release the epc 10 usb the newest member of the epc 10 family of fully. Unlike other patch clamp amplifiers, the model 2400 has a voltage follower in the probe. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is equivalent to a fully equipped setup, which includes a patch. No selects the amplifier channel displayed on the panel when a multichannel amplifier is in use. The epc 10 usb patch clamp amplifier is available with either one epc 10 usb, two epc 10 usb double, three epc 10 usb triple or four epc 10 usb quadro integrated amplifier modules. The downscaling is necessary to avoid saturation of the lockin amplifier because the output range of the epc7 is 10 v, whereas the maximum input range of the lockin amplifier is only. Three major advantages em ployed in the design of the epc 10 usb are. Epc 10 usb single patch clamp amplifiers, potentiostats. Epc 10 usb patch clamp amplifier family epc 10 usb double, triple, quadro patch clamp amplifier the epc 10 usb double, triple and quadro amplifiers are the optimal instruments for performing multiple patch cell experiments. The novel program design of patch master is perfectly suited for the operation of multichannel stimulation as used for patch clamp amplifiers with multiple headstages e. Heka elektronik patch clamp amplifiers, potentiostats. Sigworth department of cellular and molecular physiology, yale university school of medicine, 333 cedar street, new haven, ct 06510, usa received 30 march 1994. Each amplifier is controlled independently through heka patchmaster software.
Although either two epc 10 usb double, three epc 10 usb triple or four epc 10 usb quadro amplifiers are. It helps in current clamp recordings, field potential recordings in ion channel and highspeed current clamp recording within the same head stage. Apply the cfast fastcapacitance correction, wait for the seal to stabilize between 5 10 g. The software of patchmaster is also much better, in my opinion. The epc 10 usb patch clamp amplifiers are successors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990, and the well known. Although two, three or four amplifiers are combined in a single housing, along with. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster or patchmaster next software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system. Novel aspects of the circuit design of this instrument are discussed, with. Epc 10 usb heka patch clamp amplifiers warner instruments. For the most uptodate information on warner instruments news and technologies, signup for our newsletter. Although either two epc 10 usb double, three epc 10 usb triple or four epc 10 usb quadro amplifiers are combined in a single housing. It has neither frontpanel controls nor internal trim adjustments.
Nanion portapatch planar patch clamping automate scientific. Amplifier for voltage clamping using patch electrodes on. The advanced integration of the epc 10 usb with the interface and software minimizes total recording noise, eliminates compatibility problems and reduces additional. This amplifier is truly a unique hybrid patch clamp amplifier with its design and featureset primarily based upon the manually controlled epc 8. Admittancebased measurement of membrane capacitance using. The heka epc10 usb patch clamp amplifiers are successors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990, and the wellknown epc 10 patch clamp amplifiers, which replaced the epc 9s in 2002. Heka epc10 usb patch clamp amplifier online at digitimer uk. The porta patch is routinely provided with an axonmdc multiclamp amplifier or an epc 10 amplifier from heka together with a computer and the software packages. The epc 10 usb patch clamp amplifiers are successors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990, and the well known epc 10 patch clamp amplifiers, which replaced the epc 9s in 2002.
Patch clamp recordings in the whole cell configuration, and single channel recordings in the cell attached configuration, can be performed with planar patch clamp chips. Heka elektronik epc 10 single patch clamp amplifier good working condition, was removed by us from a large pharma lab that concentrated on the electrophysiology studies. Heka elektronik epc 10 usb hardware manual pdf download. Heka is pleased to release the newest family of fully computer controlled patch clamp amplifiers. Design of the epc9, a computercontrolled patchclamp. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is a fully equipped recording setup. For the screening of pharmaceutical compounds or the characterization of expressed channels, highthroughput patchclamp systems are being developed, where recordings are made from cells in each well of a 96 or 384well plate. The elc03xs amplifier is the only commercially available amplifier that is not only designed for the loose patch clamp technique for recording in slice or in vivo preparations, but also for a wide range of other electrophysiological experiments. Current injection path via the injection capacitor. Epc 10 usb patch clamp amplifier family electrophysiology models and features epc 10 usb single patch clamp amplifier the epc 10 is a complete data acquisition system, which can be used with hekas patchmaster or tida software. Small molecules for early endosomespecific patch clamping. The epc 9 computercontrolled amplifier has no frontpanel controls.
Heka elektronik single patch clamp amplifier epc 10 epc10. There are many advantages to a fully digitallycontrolled patch clamp amplifier. Epc 10 usb single patch clamp amplifier epc 10 usb double triple quadro patch clamp amplifiers data acquisition ced data acquisition and analysis ced power140 amplifier ced micro140 amplifier ced 1902 isolated pre amplifier in vitro measystems axion biosystems. The portfolio of amplifiers includes axopatch 200b for ultra lownoise singlechannel recordings, multiclamp 700b for wholecell voltage clamp and highspeed current clamp recordings. The unique feature of this virtual instrument is that it is thoroughly integrated with a computercontrolled patch clamp amplifier epc 9 to allow estimation of equivalent circuit parameters based upon calibrated admittance. The epc10 is well suited for both voltage and current clamp.
The epc 7 represents roughly the seventh in the series of patch. Axon instruments appears to be the gold standard theyre the. The epc 10 usb patch clamp amplifiers are succes sors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990123, and the well known epc 10 patch clamp amplifiers, which replaced the epc 9s in 2002. Heka elektronik download the latest software release.
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